Navitar’s Video ZFL Scope is a Macro/Micro fluorescence vision
system that utilizes interchangeable professional fluorescent cubes and
internal focus to create an image compatible with most existing camera
systems. It is a simple means of doing very sophisticated, task oriented
fluorescence without the expense and complexity associated with a fully
loaded research microscope.
Utilizes interchangeable professional fluorescence cubes to create
images compatible with most existing camera systems.
Much lower cost and smaller (more compact) than a traditional microscope.
Field of view up to 9.2 mm with a 2/3” CCD in macro mode.
Micro mode utilizes professional infinity corrected objectives.
Remote UV light source includes a long-life metal halide bulb.
Zoom and fixed systems available. Fine focus included with both systems.
Basic Components of Any Video Fluorescence System
A light source emitting the wavelengths required
to cause the labeling dye to fluorescence. Two different remote light
sources are available, a halogen light for the longer wavelengths, and
a metal arc lamp for the UV. LED illumination is currently under development.
An integrated cube that optimizes performance by
stopping all but the desired (excitation) wavelength from reaching the
object and then stopping all but the fluorescing wavelength (emitting)
from reaching the camera. There
is a multitude of off-the-shelf cubes available depending on which labeling
dye is being used.
The Navitar system permits the usage of all standard Olympus BX2 Fluorescent
Cubes, which are available from multiple sources. These are captured
singularly in a quick change holder requiring only a minute to interchange.
Information on filter sets and cubes can be found at www.chroma.com.
A camera whose sensitivity and bandwidth are adequate
to handle the fluorescing light levels (which can sometimes be minimal).
All other components are optics and mechanics that permit variation
of the magnification (field of view) and resolution, along with a means
of achieving critical focus.
Navitar System is Suitable for Operation in Macro or Micro Mode
Macro Mode
In the Macro mode, a fixed lens system provides up to a 9.2 mm diameter
field of view at a 165 mm working distance with a 2/3" format camera.
A 6.5X zoom accessory provides up to a 15 mm diameter field at a 108 mm
working distance. Various lens attachments allow the working distance
to change from 32 mm to 165 mm. An add-on focus accessory provides a means
of adjusting focus without requiring a microscope type stand.
Micro Mode
In the Micro mode, the same single lens system provides a 0.9X coupling
of any standard 200 mm tube length, infinity corrected, fluorescent microscope
objective to the camera.
OEM customers creating an automated system for scanning and post processing
of proteomics.
Production laboratory using image processing software to count and
categorize selected images.
High school or university professor, on a limited budget, who needs
to demonstrate certain fluorescent characteristics to a group.
A field operator who needs instant results involving agriculture,
oceanography, or criminology.
Museum or aquarium setting-up a public display.
Research scientists in government laboratories.
Ideal Uses for the Navitar Video ZFL Scope
Transgenic organisms.
All GFP (Green Fluorescent Protein) studies involving living
cells and tissues: C. Elegans, D. Melanogaster, D. Rerio Larva,
Zebra Fish, Oocytes, Nematodes, Drosophilia.
is a multitude of off-the-shelf cubes available depending on which labeling
dye is being used.
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